中国科学院化学所李峻柏团队报道了基于ATP合成酶重组纳米结构的光酶催化ATP生成。相关研究成果发表在2023年8月22日出版的《美国化学会杂志》。

研究人员证明了ATP合酶在微胶囊表面重构的蛋白脂质体涂层可以实现光酶催化的氧化磷酸化。通过半导体石墨氮化碳（g-C3N4）纳米片和聚电解质的逐层沉积组装微胶囊。研究发现，来自聚电解质的电子转移到g-C3N4纳米片上，这增强了光生电子-空穴对的分离。因此，包封的g-C3N4纳米片作为光酶在光照下加速葡萄糖氧化为葡萄糖酸以产生质子。建立向外跨膜质子梯度以驱动ATP合酶合成三磷酸腺苷。

通过这样的组装系统，实现了光驱动的氧化磷酸化。这表明组装的光酶可以用于氧化磷酸化，为化学能量转化为生物能量创造了一种不同寻常的方式。与传统的氧化磷酸化系统相比，这种人工设计能够实现更高的能量转换效率。

附：英文原文

Title: Photozyme-Catalyzed ATP Generation Based on ATP Synthase-Reconstituted Nanoarchitectonics

Author: Zibo Li, Fanchen Yu, Xia Xu, Tonghui Wang, Jinbo Fei, Jingcheng Hao, Junbai Li

Issue&Volume: August 22, 2023

Abstract: We demonstrate that ATP synthase-reconstituted proteoliposome coatings on the surface of microcapsules can realize photozyme-catalyzed oxidative phosphorylation. The microcapsules were assembled through layer-by-layer deposition of semiconducting graphitic carbon nitride (g-C3N4) nanosheets and polyelectrolytes. It is found that electrons from polyelectrolytes are transferred to g-C3N4 nanosheets, which enhances the separation of photogenerated electron–hole pairs. Thus, the encapsulated g-C3N4 nanosheets as the photozyme accelerate oxidation of glucose into gluconic acid to yield protons under light illumination. The outward transmembrane proton gradient is established to drive ATP synthase to synthesize adenosine triphosphate. With such an assembled system, light-driven oxidative phosphorylation is achieved. This indicates that an assembled photozyme can be used for oxidative phosphorylation, which creates an unusual way for chemical-to-biological energy conversion. Compared to conventional oxidative phosphorylation systems, such an artificial design enables higher energy conversion efficiency.

DOI: 10.1021/jacs.3c06090

Source: https://pubs.acs.org/doi/10.1021/jacs.3c06090