美国斯坦福大学Jonathan Z. Long研究小组绘制出小鼠运动训练的全机体、细胞类型特异性分泌组图谱。相关论文于2023年5月3日在线发表在《细胞—代谢》杂志上。

研究人员使用细胞类型特异性的蛋白质组学方法，生成了21个细胞类型、10个组织的小鼠运动训练调节分泌物组图。这些数据集确定了超过200个运动训练调节的细胞类型-分泌的蛋白质对，其中大部分以前没有被报道过。Pdgfra-cre标记的分泌体对运动训练的反应最强烈。最后，研究人员显示了细胞内羧酸酯酶的蛋白形式具有抗肥胖、抗糖尿病和提高运动能力的活性，这些蛋白从肝脏中的分泌被运动训练所诱导。

据了解，人们对确定介导组织交流的血源性因素和作为体育活动分子效应物的功能有很大兴趣。虽然过去的研究集中在单个分子或细胞类型上，但整个机体对体育活动的分泌组反应还没有被评估。

附：英文原文

Title: Organism-wide, cell-type-specific secretome mapping of exercise training in mice

Author: Wei Wei, Nicholas M. Riley, Xuchao Lyu, Xiaotao Shen, Jing Guo, Steffen H. Raun, Meng Zhao, Maria Dolores Moya-Garzon, Himanish Basu, Alan Sheng-Hwa Tung, Veronica L. Li, Wentao Huang, Amanda L. Wiggenhorn, Katrin J. Svensson, Michael P. Snyder, Carolyn R. Bertozzi, Jonathan Z. Long

Issue&Volume: 2023-05-03

Abstract: There is a significant interest in identifying blood-borne factors that mediate tissuecrosstalk and function as molecular effectors of physical activity. Although paststudies have focused on an individual molecule or cell type, the organism-wide secretomeresponse to physical activity has not been evaluated. Here, we use a cell-type-specificproteomic approach to generate a 21-cell-type, 10-tissue map of exercise training-regulatedsecretomes in mice. Our dataset identifies >200 exercise training-regulated cell-type-secretedprotein pairs, the majority of which have not been previously reported. Pdgfra-cre-labeled secretomes were the most responsive to exercise training. Finally, weshow anti-obesity, anti-diabetic, and exercise performance-enhancing activities forproteoforms of intracellular carboxylesterases whose secretion from the liver is inducedby exercise training.

DOI: 10.1016/j.cmet.2023.04.011

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(23)00138-9