北京大学邓宏魁等研究人员合作通过化学重编程高效、快速产生人类多能干细胞。相关论文于2023年3月20日在线发表在《细胞—干细胞》杂志上。

研究人员最近展示了用化学方法将人类体细胞重新编程为多能干细胞（hCiPSC），这为细胞命运的操纵提供了一种强有力的方法。然而，这种化学方法的效用目前受到缓慢动力学的阻碍。通过筛选小分子助推剂和系统地优化原始条件，研究人员建立了一个强大的、化学定义的重编程方案，它大大缩短了诱导时间，从约50天缩短至16天的时间，并能从所有17个测试的供体中高度可重复和有效地生成hCiPSC。

研究人员发现，这种优化的方案通过在早期阶段促进细胞增殖和氧化磷酸化代谢活动，使重编程过程更加直接。这些结果突出了一种独特的化学重编程途径，它导致了生成人类多能干细胞的捷径，这代表了人类细胞命运操纵的一种强大策略。

附：英文原文

Title: Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming

Author: Shijia Liuyang, Guan Wang, Yanglu Wang, Huanjing He, Yulin Lyu, Lin Cheng, Zhihan Yang, Jingyang Guan, Yao Fu, Jialiang Zhu, Xinxing Zhong, Shicheng Sun, Cheng Li, Jinlin Wang, Hongkui Deng

Issue&Volume: 2023-03-20

Abstract: We recently demonstrated the chemical reprogramming of human somatic cells to pluripotentstem cells (hCiPSCs), which provides a robust approach for cell fate manipulation.However, the utility of this chemical approach is currently hampered by slow kinetics.Here, by screening for small molecule boosters and systematically optimizing the originalcondition, we have established a robust, chemically defined reprogramming protocol,which greatly shortens the induction time from ～50 days to a minimum of 16 days andenables highly reproducible and efficient generation of hCiPSCs from all 17 testeddonors. We found that this optimized protocol enabled a more direct reprogrammingprocess by promoting cell proliferation and oxidative phosphorylation metabolic activitiesat the early stage. Our results highlight a distinct chemical reprogramming pathwaythat leads to a shortcut for the generation of human pluripotent stem cells, whichrepresents a powerful strategy for human cell fate manipulation.

DOI: 10.1016/j.stem.2023.02.008

Source: https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00069-3