华中师范大学化朱成周团队报道了水活化促进电化学发光。相关研究成果发表在2023年3月8日出版的《德国应用化学》。

在传统的鲁米诺电化学发光（ECL）系统中，过氧化氢和溶解氧被用作典型的共反应物产生活性氧（ROS），以实现高效的ECL。然而，过氧化氢的自分解和氧在水中的有限溶解度不可避免地限制了鲁米诺ECL系统的检测精度和发光效率。

受ROS介导的ECL机制的启发，研究人员首次使用钴-铁层状双氢氧化物作为共反应加速器，有效地活化水以生成ROS，以增强鲁米诺的发光。实验研究证实了在电化学水氧化过程中羟基和超氧化物自由基的形成，这些自由基随后与鲁米诺阴离子自由基反应以触发强ECL信号。最后，碱性磷酸酶的检测已成功实现，具有令人印象深刻的灵敏度和重复性，可用于实际样品分析。

附：英文原文

Title: Water Activation for Boosting Electrochemiluminescence

Author: Mengzhen Xi, Zhichao Wu, Zhen Luo, Ling Ling, Weiqing Xu, Runshi Xiao, Hengjia Wang, Qie Fang, Liuyong Hu, Wenling Gu, Chengzhou Zhu

Issue&Volume: 2023-03-08

Abstract: In conventional luminol electrochemiluminescence (ECL) systems, hydrogen peroxide and dissolved oxygen are employed as typical co-reactants to produce reactive oxygen species (ROS) for efficient ECL emission. However, the self-decomposition of hydrogen peroxide and limited solubility of oxygen in water inevitably restrict the detection accuracy and luminous efficiency of luminol ECL system. Inspired by ROS-mediated ECL mechanism, for the first time, we used cobalt-iron layered double hydroxide as co-reaction accelerator to efficiently activate water to generate ROS for enhancing luminol emission. Experimental investigations verify the formation of hydroxyl and superoxide radicals in the process of electrochemical water oxidation, which subsequently react with luminol anion radicals to trigger strong ECL signals. Finally, the detection of alkaline phosphatase has been successfully achieved with impressive sensitivity and reproducibility for practical sample analysis.

DOI: 10.1002/anie.202302166

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202302166