上海交通大学周永丰团队报道了人工细胞骨架的膜结合内生长及其选择性拆卸。相关研究成果发表在2022年4月19日出版的《德国应用化学》。

细胞骨架是最重要的细胞组成之一。到目前为止，大多数报道的人工细胞骨架都是基于囊泡凝胶策略。

该文中，研究人员报道了一种膜结合的内向生长途径，以制备由包膜生长而来的细胞骨架样和径向排列的纳米纤维，以获得膜结合的人工细胞骨架(MACs)。通过直接观察干燥和液态的中间体，研究人员揭示了其中的机理。此外，在还原剂存在的情况下，制备的MACs表现出选择性拆卸行为：包膜和MACs都可以拆卸，或者只有MACs可以通过选择性地引入动态二硫键（DS）并通过紫外线（UV）照射的开关来拆卸。

该研究为构建成分和方向可控的人工细胞骨架提供了一种新的分层自组装方法。

附：英文原文

Title: Membrane-Bound Inward-Growth of Artificial Cytoskeletons and Their Selective Disassembly

Author: Qingsong Xu, Shanlong Li, Meiwei Qi, Jing Gao, Chuanshuang Chen, Pei Huang, Yuling Wang, Chunyang Yu, Wei Huang, Yongfeng Zhou

Issue&Volume: 2022-04-19

Abstract: Cytoskeleton is one of the most important cellular components. Up to now, most of the reported artificial cytoskeletons are based on a gel-in-vesicle strategy. Herein, we report a membrane-bound inward-growth pathway to prepare cytoskeleton-like and radially aligned nanofibers grown from capsule membranes to get membrane-bound artificial cytoskeletons (MACs). The mechanism therein is disclosed thorough the direct observation of the intermediates in both dried and liquid states. Furthermore, the as-prepared MACs show a selective disassembly behavior in the presence of reductants: both capsule membranes and MACs can be disassembled or only MACs can be disassembled through the selective introduction of dynamic disulfide bonds (DS) into them and by the switch of ultraviolet (UV) irradiation. The present work has provided a new hierarchical self-assembly way to construct artificial cytoskeletons with controlled compositions and orientations.

DOI: 10.1002/anie.202204440

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202204440