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细胞内Ca2+释放对树突特征选择性的隔室特异性调节
作者:小柯机器人 发布时间:2022/3/20 16:21:12

美国哥伦比亚大学Attila Losonczy和Franck Polleux共同合作取得新的研究进展。他们研究发现了细胞内Ca2+释放对树突特征选择性的隔室特异性调节。该项研究成果2022年3月18日在线发表于《科学》杂志上。

在这里,研究人员结合了单个 CA1 锥体神经元的电穿孔、空间导航期间树突和体细胞活动的同时成像、光遗传学位置场诱导和 ICR 细胞溶质影响的急性遗传增强,以揭示 ICR 支持树突特征选择性的建立和形成确定输出级接收域的综合属性。ICR 的这种作用在顶端比在基部树突中更为突出。他们认为,ICR与体内电路级架构合作,以特定隔间的方式促进行为相关可塑性的出现。

据介绍,树突状钙信号传递是使动物适应环境的神经可塑性机制的核心。长期以来,人们一直认为内质网的细胞内钙释放 (ICR) 会影响这些机制。然而,ICR尚未在哺乳动物神经元体内进行研究。

附:英文原文

Title: Compartment-specific tuning of dendritic feature selectivity by intracellular Ca2+ release

Author: Justin K. O’Hare, Kevin C. Gonzalez, Stephanie A. Herrlinger, Yusuke Hirabayashi, Victoria L. Hewitt, Heike Blockus, Miklos Szoboszlay, Sebi V. Rolotti, Tristan C. Geiller, Adrian Negrean, Vikas Chelur, Franck Polleux, Attila Losonczy

Issue&Volume: 2022-03-18

Abstract: Dendritic calcium signaling is central to neural plasticity mechanisms that allow animals to adapt to the environment. Intracellular calcium release (ICR) from the endoplasmic reticulum has long been thought to shape these mechanisms. However, ICR has not been investigated in mammalian neurons in vivo. We combined electroporation of single CA1 pyramidal neurons, simultaneous imaging of dendritic and somatic activity during spatial navigation, optogenetic place field induction, and acute genetic augmentation of ICR cytosolic impact to reveal that ICR supports the establishment of dendritic feature selectivity and shapes integrative properties determining output-level receptive fields. This role for ICR was more prominent in apical than in basal dendrites. Thus, ICR cooperates with circuit-level architecture in vivo to promote the emergence of behaviorally relevant plasticity in a compartment-specific manner.

DOI: abm1670

Source: https://www.science.org/doi/10.1126/science.abm1670#con13

 

期刊信息
Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037