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氮对氮酶活性的抑制涉及对大豆根瘤中细胞质转化酶的调节
作者:小柯机器人 发布时间:2024/7/3 22:24:53

广州大学关跃峰团队发现,氮对氮酶活性的抑制涉及对大豆根瘤中细胞质转化酶的调节。该项研究成果于2024年6月29日在线发表在《遗传学报》上。

研究人员发现编码细胞质转化酶的GmCIN1是对蔗糖在根瘤中的利用进行氮调节的途径。GmCIN1在成熟的大豆根瘤中富集,其表达受氮状态的调控。利用基因组编辑技术敲除GmCIN1可部分模拟氮对氮酶活性和糖含量的抑制作用,以及高氮对根瘤转录组的影响。这表明GmCIN1部分介导了高氮对根瘤活性的抑制作用。
 
此外,ChIP-qPCR和EMSA发现SNAP1/2转录因子直接与GmCIN1启动子结合。此外,SNAP1/2可能参与了高浓度氮对成熟根瘤中GmCIN1表达的抑制。这些研究结果为了解氮信号调节因子对碳代谢基因的转录调控参与氮诱导的氮酶活性抑制提供了启示。

研究人员表示,豆科植物的共生固氮作用(SNF)受到土壤中无机氮的抑制。高氮对氮酶活性的抑制与碳在根瘤中的分配和代谢被剥夺有关。然而,其潜在的分子机制仍不清楚。

附:英文原文

Title: Nitrogen inhibition of nitrogenase activity involves the modulation of cytosolic invertase in soybean nodule

Author: Yuefeng Guan c

Issue&Volume: 2024/06/29

Abstract: Legume symbiotic nitrogen fixation (SNF) is suppressed by inorganic N in the soil. High N inhibition of nitrogenase activity is associated with the deprivation of carbon allocation and metabolism in nodules. However, the underlying molecular mechanisms remain unclear. Here, we identify GmCIN1 which encodes a cytosolic invertase, as a gateway for the N-tuning of sucrose utilization in nodules. GmCIN1 is enriched in mature soybean nodules and its expression is regulated by nitrogen status. The knockout of GmCIN1 using genome editing partially mimicks the inhibitory effects of N on nitrogenase activity and sugar content and the impact of high N on nodule transcriptomes. This indicates that GmCIN1 partially mediates the high N inhibition of nodule activity. Moreover, ChIP-qPCR and EMSA reveal that SNAP1/2 transcription factors directly bind to the GmCIN1 promoter. In addition, SNAP1/2 may be involved in the repression of GmCIN1 expression in mature nodules at high N concentrations. Our findings provide insights into the involvement of the transcriptional tuning of C metabolism genes by N-signaling modulators in the N-induced inhibition of nitrogenase activity.

DOI: 10.1016/j.jgg.2024.06.013

Source: https://www.sciencedirect.com/science/article/abs/pii/S1673852724001553

期刊信息

Journal of Genetics and Genomics《遗传学报》,创刊于1974年。隶属于爱思唯尔出版集团,最新IF:5.9

官方网址:https://www.sciencedirect.com/journal/journal-of-genetics-and-genomics
投稿链接:https://www2.cloud.editorialmanager.com/jgg/default2.aspx