近日，德国海德堡大学Sascha Dietrich等研究人员合作发现，多模态和空间分辨分析确定结节性B细胞淋巴瘤实体中T细胞浸润的不同模式。相关论文于2024年2月20日在线发表在《自然—细胞生物学》杂志上。

研究人员基于转录组和表位的细胞索引、T细胞受体测序、流式细胞术和多重免疫荧光技术，对弥漫大B细胞淋巴瘤、套细胞淋巴瘤、滤泡淋巴瘤或边缘区淋巴瘤患者的101个淋巴结以及健康对照组的淋巴结绘制了结节性B细胞非霍奇金淋巴瘤（B-NHL）的深度T细胞参考图谱。这种多模态资源揭示了T细胞微环境在B-NHL实体间和实体内的定量和空间畸变。

PD1⁺ TCF7^-细胞毒性T细胞、T滤泡辅助细胞或IKZF3⁺调节性T细胞的数量差异与其克隆扩增有关。PD1⁺ TCF7^-细胞毒性T细胞的大量存在与生存率低有关。这项研究以前所未有的全面性描述了淋巴瘤浸润T细胞，为研究淋巴瘤生物学和预后提供了独特的资源。

据了解，在B-NHL中，T细胞的重新定向已成为一项极具吸引力的治疗原则。然而，目前还缺乏对不同B-NHL实体淋巴瘤浸润T细胞的详细描述。

附：英文原文

Title: Multimodal and spatially resolved profiling identifies distinct patterns of T cell infiltration in nodal B cell lymphoma entities

Author: Roider, Tobias, Baertsch, Marc A., Fitzgerald, Donnacha, Vhringer, Harald, Brinkmann, Berit J., Czernilofsky, Felix, Knoll, Mareike, Lla-Cid, Laura, Mathioudaki, Anna, Fabender, Bianca, Herbon, Maxime, Lautwein, Tobias, Bruch, Peter-Martin, Liebers, Nora, Schrch, Christian M., Passerini, Verena, Seifert, Marc, Brobeil, Alexander, Mechtersheimer, Gunhild, Mller-Tidow, Carsten, Weigert, Oliver, Seiffert, Martina, Nolan, Garry P., Huber, Wolfgang, Dietrich, Sascha

Issue&Volume: 2024-02-20

Abstract: The redirection of T cells has emerged as an attractive therapeutic principle in B cell non-Hodgkin lymphoma (B-NHL). However, a detailed characterization of lymphoma-infiltrating T cells across B-NHL entities is missing. Here we present an in-depth T cell reference map of nodal B-NHL, based on cellular indexing of transcriptomes and epitopes, T cell receptor sequencing, flow cytometry and multiplexed immunofluorescence applied to 101 lymph nodes from patients with diffuse large B cell, mantle cell, follicular or marginal zone lymphoma, and from healthy controls. This multimodal resource revealed quantitative and spatial aberrations of the T cell microenvironment across and within B-NHL entities. Quantitative differences in PD1+ TCF7 cytotoxic T cells, T follicular helper cells or IKZF3+ regulatory T cells were linked to their clonal expansion. The abundance of PD1+ TCF7 cytotoxic T cells was associated with poor survival. Our study portrays lymphoma-infiltrating T cells with unprecedented comprehensiveness and provides a unique resource for the investigation of lymphoma biology and prognosis.

DOI: 10.1038/s41556-024-01358-2

Source: https://www.nature.com/articles/s41556-024-01358-2