美国麻省理工学院Viktor A. Adalsteinsson，Sangeeta N. Bhatia，J. Christopher Love和Shervin Tabrizi共同合作，近期取得重要工作进展。他们研究利用引发剂瞬时降低游离DNA的清除率以改善液体活检 。相关研究成果
据介绍，液体活检能够早期发现和监测癌症等疾病，但由于血液中缺乏无细胞DNA（cfDNA）等分析物，其敏感性仍然有限。灵敏度的提高主要依赖于体外增强测序技术。
研究人员试图通过减弱体内循环肿瘤DNA（ctDNA）的清除率来短暂提高抽血样本中循环肿瘤DNA的水平。研究人员报告了两种静脉注射引发剂，在抽血前1到2小时给予，以恢复更多的ctDNA。引发剂由作用于负责cfDNA清除细胞的纳米颗粒和保护cfDNA的DNA结合抗体组成。在荷瘤小鼠中，它们大大提高了ctDNA的恢复，并提高了检测小肿瘤的灵敏度。
附：英文原文
Title: Priming agents transiently reduce the clearance of cell-free DNA to improve liquid biopsies
Author: Carmen Martin-Alonso, Shervin Tabrizi, Kan Xiong, Timothy Blewett, Sainetra Sridhar, Andjela Crnjac, Sahil Patel, Zhenyi An, Ahmet Bekdemir, Douglas Shea, Shih-Ting Wang, Sergio Rodriguez-Aponte, Christopher A. Naranjo, Justin Rhoades, Jesse D. Kirkpatrick, Heather E. Fleming, Ava P. Amini, Todd R. Golub, J. Christopher Love, Sangeeta N. Bhatia, Viktor A. Adalsteinsson
Issue&Volume: 2024-01-19
Abstract: Liquid biopsies enable early detection and monitoring of diseases such as cancer, but their sensitivity remains limited by the scarcity of analytes such as cell-free DNA (cfDNA) in blood. Improvements to sensitivity have primarily relied on enhancing sequencing technology ex vivo. We sought to transiently augment the level of circulating tumor DNA (ctDNA) in a blood draw by attenuating its clearance in vivo. We report two intravenous priming agents given 1 to 2 hours before a blood draw to recover more ctDNA. Our priming agents consist of nanoparticles that act on the cells responsible for cfDNA clearance and DNA-binding antibodies that protect cfDNA. In tumor-bearing mice, they greatly increase the recovery of ctDNA and improve the sensitivity for detecting small tumors.
DOI: adf2341
Source: https://www.science.org/doi/10.1126/science.adf2341