美国耶鲁医学院David Baddeley和Joerg Bewersdorf共同合作，近期取得重要工作进展。他们研究开发出了用于高通量纳米显微镜的集成平台。相关研究成果2023年3月13日在线发表于《自然—生物技术》杂志上。

据介绍，单分子定位显微镜可以实现几十纳米分辨率的三维荧光成像，但需要许多相机帧来重建超分辨率图像。这将典型的通量限制为每天几十个细胞。虽然帧速率现在可以提高一个数量级以上，但在现有工作流中，大数据量受到限制。

研究人员提出了一个集成的采集和分析平台，该平台利用显微镜特定的数据压缩、分布式存储和分布式分析，实现每天10000个细胞的采集与分析通量。该平台有助于在采集过程中从显微镜自动启动并远程执行图形可重构分析，甚至可以在显微镜上反馈和排队新的采集任务。研究人员通过在多孔样本格式中对每孔数百个细胞进行成像，证明了该框架的实用性。

总之，这一平台在Python-Microscopy环境（PYME）中实现，可轻松配置以控制自定义显微镜，并包含用于用户定义扩展的插件框架。

附：英文原文

Title: An integrated platform for high-throughput nanoscopy

Author: Barentine, Andrew E. S., Lin, Yu, Courvan, Edward M., Kidd, Phylicia, Liu, Miao, Balduf, Leonhard, Phan, Timy, Rivera-Molina, Felix, Grace, Michael R., Marin, Zach, Lessard, Mark, Rios Chen, Juliana, Wang, Siyuan, Neugebauer, Karla M., Bewersdorf, Joerg, Baddeley, David

Issue&Volume: 2023-03-13

Abstract: Single-molecule localization microscopy enables three-dimensional fluorescence imaging at tens-of-nanometer resolution, but requires many camera frames to reconstruct a super-resolved image. This limits the typical throughput to tens of cells per day. While frame rates can now be increased by over an order of magnitude, the large data volumes become limiting in existing workflows. Here we present an integrated acquisition and analysis platform leveraging microscopy-specific data compression, distributed storage and distributed analysis to enable an acquisition and analysis throughput of 10,000 cells per day. The platform facilitates graphically reconfigurable analyses to be automatically initiated from the microscope during acquisition and remotely executed, and can even feed back and queue new acquisition tasks on the microscope. We demonstrate the utility of this framework by imaging hundreds of cells per well in multi-well sample formats. Our platform, implemented within the PYthon-Microscopy Environment (PYME), is easily configurable to control custom microscopes, and includes a plugin framework for user-defined extensions.

DOI: 10.1038/s41587-023-01702-1

Source: https://www.nature.com/articles/s41587-023-01702-1