中国科学院深圳先进技术研究院司龙龙等研究人员利用蛋白水解靶向开发出甲型流感减毒活疫苗。相关论文于2022年7月4日在线发表在《自然—生物技术》杂志上。
Title: Generation of a live attenuated influenza A vaccine by proteolysis targeting
Author: Si, Longlong, Shen, Quan, Li, Jing, Chen, Li, Shen, Jinying, Xiao, Xue, Bai, Haiqing, Feng, Tang, Ye, Adam Yongxin, Li, Le, Zhang, Chunhe, Li, Zhen, Wang, Ping, Oh, Crystal Yuri, Nurani, Atiq, Niu, Siwen, Zhang, Chengxin, Wei, Xiaoqiong, Yuan, Wanqiong, Liao, Hao, Huang, Xiaojie, Wang, Ning, Tian, Wen-xia, Tian, Hongwei, Li, Li, Liu, Xiaoheng, Plebani, Roberto
Issue&Volume: 2022-07-04
Abstract: The usefulness of live attenuated virus vaccines has been limited by suboptimal immunogenicity, safety concerns or cumbersome manufacturing processes and techniques. Here we describe the generation of a live attenuated influenza A virus vaccine using proteolysis-targeting chimeric (PROTAC) technology to degrade viral proteins via the endogenous ubiquitin–proteasome system of host cells. We engineered the genome of influenza A viruses in stable cell lines engineered for virus production to introduce a conditionally removable proteasome-targeting domain, generating fully infective PROTAC viruses that were live attenuated by the host protein degradation machinery upon infection. In mouse and ferret models, PROTAC viruses were highly attenuated and able to elicit robust and broad humoral, mucosal and cellular immunity against homologous and heterologous virus challenges. PROTAC-mediated attenuation of viruses may be broadly applicable for generating live attenuated vaccines. Influenza virus is attenuated for vaccine production using PROTAC degradation technology.
DOI: 10.1038/s41587-022-01381-4
Source: https://www.nature.com/articles/s41587-022-01381-4
Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:31.864
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex