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Shulin蛋白通过包装轴突外动力蛋白臂来实现纤毛靶向
作者:小柯机器人 发布时间:2021/2/28 23:04:08

近日,英国MRC分子生物学实验室Andrew P. Carter及其研究组发现,Shulin蛋白通过包装轴突外动力蛋白臂来实现纤毛靶向。相关论文发表在2021年2月26日出版的《科学》杂志上。

研究人员使用四膜虫来鉴定了两个因子(Q22YU3和Q22MS1),它们结合细胞质中的动力蛋白臂(ODA),并且是将ODA传递至纤毛所必需的。Q22YU3(被命名为Shulin)将ODA运动域锁定为封闭结构并抑制了运动活动。冷冻电镜揭示了Shulin如何通过与动力蛋白尾巴结合来稳定这种紧密形式的ODA。这个发现为新组装的动力蛋白如何包装以递送至纤毛提供了分子解释。 

据介绍,真核纤毛和鞭毛中的主要力量产生者是轴突外ODA。在纤毛发生过程中,这些?1.8兆道尔顿的复合物在细胞质中组装,并通过未知机制靶向纤毛。 

附:英文原文

Title: Shulin packages axonemal outer dynein arms for ciliary targeting

Author: Girish R. Mali, Ferdos Abid Ali, Clinton K. Lau, Farida Begum, Jérme Boulanger, Jonathan D. Howe, Zhuo A. Chen, Juri Rappsilber, Mark Skehel, Andrew P. Carter

Issue&Volume: 2021/02/26

Abstract: The main force generators in eukaryotic cilia and flagella are axonemal outer dynein arms (ODAs). During ciliogenesis, these ~1.8-megadalton complexes are assembled in the cytoplasm and targeted to cilia by an unknown mechanism. Here, we used the ciliate Tetrahymena to identify two factors (Q22YU3 and Q22MS1) that bind ODAs in the cytoplasm and are required for ODA delivery to cilia. Q22YU3, which we named Shulin, locked the ODA motor domains into a closed conformation and inhibited motor activity. Cryo–electron microscopy revealed how Shulin stabilized this compact form of ODAs by binding to the dynein tails. Our findings provide a molecular explanation for how newly assembled dyneins are packaged for delivery to the cilia.

DOI: 10.1126/science.abe0526

Source: https://science.sciencemag.org/content/371/6532/910

期刊信息
Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037