科学家发现超强的人源SARS-CoV-2中和抗体
作者：小柯机器人 发布时间：2020/9/26 10:07:34
美国华盛顿大学David Veesler、瑞士Vir生物技术公司Katja Fink等研究人员合作发现超强的人源SARS-CoV-2中和抗体。这一研究成果于2020年9月24日在线发表在《科学》杂志上。
研究人员报告了两种超强的SARS-CoV-2人类中和抗体（S2E12和S2M11）的分离和鉴定，这两种抗体可保护仓鼠抵御SARS-CoV-2攻击。冷冻电镜结构显示，S2E12和S2M11竞争性地阻断ACE2的结合，并且S2M11还通过识别跨越两个相邻受体结合域的四级表位将突刺锁定在闭合构象中。包括S2M11、S2E12或先前确定的S309抗体在内的鸡尾酒疗法广泛地中和了一组循环SARS-CoV-2分离株并激活效应器功能。
这些研究结果为实施抗体鸡尾酒疗法用于预防或治疗以及避免或限制病毒逃逸突变体的出现铺平了道路。
附：英文原文
Title:Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms
Author:M. Alejandra Tortorici1,2,*, View ORCID ProfileMartina Beltramello3,*, Florian A. Lempp4, Dora Pinto
Issue&Volume:24 Sep 2020
Abstract: Efficient therapeutic options are needed to control the spread of SARS-CoV-2 that has caused more than 922,000 fatalities as of September 13th, 2020. We report the isolation and characterization of two ultrapotent SARS-CoV-2 human neutralizing antibodies (S2E12 and S2M11) that protect hamsters against SARS-CoV-2 challenge. Cryo-electron microscopy structures show that S2E12 and S2M11 competitively block ACE2 attachment and that S2M11 also locks the spike in a closed conformation by recognition of a quaternary epitope spanning two adjacent receptor-binding domains. Cocktails including S2M11, S2E12 or the previously identified S309 antibody broadly neutralize a panel of circulating SARS-CoV-2 isolates and activate effector functions. Our results pave the way to implement antibody cocktails for prophylaxis or therapy, circumventing or limiting the emergence of viral escape mutants.
DOI:DOI: 10.1126/science.abe3354
Source: https://science.sciencemag.org/content/early/2020/09/23/science.abe3354
期刊信息
Science：《科学》，创刊于1880年。隶属于美国科学促进会，最新IF：41.037