美国加州大学Mazen A. Kheirbek研究组取得最新进展。他们揭示了腹侧海马（vHPC）网络的回路和分子结构。相关论文发表在2020年9月14日出版的《自然-神经科学》杂志上。

他们使用病毒和测序方法来定义扩展腹侧CA1（vCA1）回路的逻辑。使用遗传条形码神经元（MAPseq）的高通量测序来绘制成千上万个vCA1神经元的轴突投影，他们确定了一群神经元，它们同时向多个已知区域扩散信息，以调节压力轴和避免接触行为。通过vCA1投射神经元的分子谱分析和病毒输入-输出示踪，他们展示了具有不同投射靶标的神经元在输入和转录特征上如何表现不同。

这些研究揭示了vCA1新的组织原则，可能是其功能异质性的基础。

研究人员表示，vHPC是处理情感信息的网络中的重要枢纽。尽管最近的研究表明vCA1投射神经元在功能上是可分离的，但如何组织vCA1的输入和输出的基本原理仍不清楚。

附：英文原文

Title: Circuit and molecular architecture of a ventral hippocampal network

Author: Mark M. Gergues, Kasey J. Han, Hye Sun Choi, Brandon Brown, Kelsey J. Clausing, Victoria S. Turner, Ilia D. Vainchtein, Anna V. Molofsky, Mazen A. Kheirbek

Issue&Volume: 2020-09-14

Abstract: The ventral hippocampus (vHPC) is a critical hub in networks that process emotional information. While recent studies have indicated that ventral CA1 (vCA1) projection neurons are functionally dissociable, the basic principles of how the inputs and outputs of vCA1 are organized remain unclear. Here, we used viral and sequencing approaches to define the logic of the extended vCA1 circuit. Using high-throughput sequencing of genetically barcoded neurons (MAPseq) to map the axonal projections of thousands of vCA1 neurons, we identify a population of neurons that simultaneously broadcast information to multiple areas known to regulate the stress axis and approach–avoidance behavior. Through molecular profiling and viral input–output tracing of vCA1 projection neurons, we show how neurons with distinct projection targets may differ in their inputs and transcriptional signatures. These studies reveal new organizational principles of vCA1 that may underlie its functional heterogeneity.

DOI: 10.1038/s41593-020-0705-8

Source: https://www.nature.com/articles/s41593-020-0705-8