德国柏林工业大学Juri Rappsilber、欧洲分子生物学实验室Julia Mahamid等研究人员，合作解析了活跃转录-翻译表达组的细胞内结构。相关论文发表在2020年7月31日出版的《科学》杂志上。

Author: Francis J. O’Reilly, Liang Xue, Andrea Graziadei, Ludwig Sinn, Swantje Lenz, Dimitry Tegunov, Cedric Bltz, Neil Singh, Wim J. H. Hagen, Patrick Cramer, Jrg Stülke, Julia Mahamid, Juri Rappsilber

Issue&Volume: 2020/07/31

Abstract: Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae. We combined whole-cell cross-linking mass spectrometry, cellular cryo–electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell.

DOI: 10.1126/science.abb3758

Source: https://science.sciencemag.org/content/369/6503/554