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科学家发现新的新冠病毒中和抗体
作者：小柯机器人 发布时间：2020/6/24 13:37:56

中国军事医学科学院陈薇、李建民、西湖大学周强等研究人员合作发现一个结合SARS-CoV-2突刺蛋白N末端结构域的中和性人源抗体。该研究于2020年6月22日在线发表于《科学》。

研究人员从十名恢复期的COVID-19患者中分离并鉴定了单克隆抗体（mAb）。三个单克隆抗体显示出对真正SARS-CoV-2的中和活性。一个名为4A8的mAb对真正和假型SARS-CoV-2均显示出高中和效力，但不结合受体结合结构域（RBD）。
 
通过确定其与突刺（S）蛋白复合的冷冻电镜结构，整体分辨率为3.1埃和局部界面分辨率为3.3埃，研究人员将4A8的表位定义为S蛋白的N末端结构域（NTD） 。这表明NTD是治疗性mAb靶向COVID-19的有望靶标。
 
据悉，研发抗SARS-CoV-2的疗法可以通过抗原表位的分布来指导，不仅限于突S蛋白的RBD，还包括整个S蛋白。
 
附：英文原文
 
Title: A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2

Author: Xiangyang Chi, Renhong Yan, Jun Zhang, Guanying Zhang, Yuanyuan Zhang, Meng Hao, Zhe Zhang, Pengfei Fan, Yunzhu Dong, Yilong Yang, Zhengshan Chen, Yingying Guo, Jinlong Zhang, Yaning Li, Xiaohong Song, Yi Chen, Lu Xia, Ling Fu, Lihua Hou, Junjie Xu, Changming Yu, Jianmin Li, Qiang Zhou, Wei Chen

Issue&Volume: 2020/06/22

Abstract: Abstract Developing therapeutics against SARS-CoV-2 could be guided by the distribution of epitopes, not only on the receptor binding domain (RBD) of the Spike (S) protein, but also across the full Spike (S) protein. We isolated and characterized monoclonal antibodies (mAbs) from ten convalescent COVID-19 patients. Three mAbs showed neutralizing activities against authentic SARS-CoV-2. An mAb, named 4A8, exhibits high neutralization potency against both authentic and pseudotyped SARS-CoV-2, but does not bind the RBD. We defined the epitope of 4A8 as the N terminal domain (NTD) of the S protein by determining its cryo-EM structure in complex with the S protein to an overall resolution of 3.1 Angstrom and local resolution of 3.3 Angstrom for the 4A8-NTD interface. This points to the NTD as a promising target for therapeutic mAbs against COVID-19.

DOI: 10.1126/science.abc6952

Source: https://science.sciencemag.org/content/early/2020/06/19/science.abc6952

期刊信息
Science：《科学》，创刊于1880年。隶属于美国科学促进会，最新IF：41.037