德国马普研究所Ilme Schlichting研究组报道了飞秒串联晶体学超快泵-探针实验的激发指南。2020年5月25日，《自然—方法学》在线发表了这一成果。

研究人员报道了数值和实验方法来确定微晶中单光子激发需要多少个光子，同时考虑了散射造成的损失。

据了解，利用X射线自由电子激光器进行时间分辨的晶体学能够在超快的时间尺度上对光诱导的反应进行结构表征。为了具有生物学和化学相关性，这些研究必须在适当的光激发方法下进行，以避免多光子伪影，这是最近研究中的常见问题。

附：英文原文

Title: Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography

Author: Marie Luise Grnbein, Miriam Stricker, Gabriela Nass Kovacs, Marco Kloos, R. Bruce Doak, Robert L. Shoeman, Jochen Reinstein, Sylvain Lecler, Stefan Haacke, Ilme Schlichting

Issue&Volume: 2020-05-25

Abstract: Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.

DOI: 10.1038/s41592-020-0847-3

Source: https://www.nature.com/articles/s41592-020-0847-3