美国加州大学Joseph Bondy-Denomy研究团队近期取得新进展。他们回顾了簇状、规则间隔的短回文重复序列（CRISPR）和 CRISPR相关（Cas）系统的蛋白抑制子抗CRISPR（Acr）蛋白的应用：CRISPR-Cas技术的天然制动器。该项研究成果发表在2020年3月16日出版的《自然-方法学》杂志上。

Acr蛋白的发现使人们能够开发出更加可控和精确的CRISPR-Cas工具。研究组讨论了Acr蛋白在原核和哺乳动物细胞、生物体和生态系统中对CRISPR-Cas系统进行翻译后调控的应用。

据介绍， CRISPR和Cas基因是一种多样的原核适应性免疫系统家族，已成为一种生物技术工具和治疗方法。

附：英文原文

Title: Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies

Author: Nicole D. Marino, Rafael Pinilla-Redondo, Blint Csrg, Joseph Bondy-Denomy

Issue&Volume: 2020-03-16

Abstract: Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.

DOI: 10.1038/s41592-020-0771-6

Source: https://www.nature.com/articles/s41592-020-0771-6