比利时鲁汶大学Peter Carmeliet、丹麦奥胡斯大学Yonglun Luo、中国中山大学Xuri Li等研究人员合作绘制了小鼠内皮细胞（EC）的单细胞转录组图谱。2020年2月13日，国际知名学术期刊《细胞》在线发表了这一成果。
Title: Single-Cell Transcriptome Atlas of Murine Endothelial Cells
Author: Joanna Kalucka, Laura P.M.H. de Rooij, Jermaine Goveia, Katerina Rohlenova, Sébastien J. Dumas, Elda Meta, Nadine V. Conchinha, Federico Taverna, Laure-Anne Teuwen, Koen Veys, Melissa García-Caballero, Shawez Khan, Vincent Geldhof, Liliana Sokol, Rongyuan Chen, Lucas Treps, Mila Borri, Pauline de Zeeuw, Charlotte Dubois, Tobias K. Karakach, Kim D. Falkenberg, Magdalena Parys, Xiangke Yin, Stefan Vinckier, Yuxiang Du, Robert A. Fenton, Luc Schoonjans, Mieke Dewerchin, Guy Eelen, Bernard Thienpont, Lin Lin, Lars Bolund, Xuri Li, Yonglun Luo, Peter Carmeliet
Issue&Volume: February 13, 2020
Abstract: The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried.We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissuesand identified 78 EC subclusters, including Aqp7+ intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis,liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressedpartially overlapping marker genes. Arterial, venous, and lymphatic ECs shared moremarkers in more tissues than did heterogeneous capillary ECs. ECs from different vascularbeds (arteries, capillaries, veins, lymphatics) exhibited transcriptome similarityacross tissues, but the tissue (rather than the vessel) type contributed to the ECheterogeneity. Metabolic transcriptome analysis revealed a similar tissue-groupingphenomenon of ECs and heterogeneous metabolic gene signatures in ECs between tissuesand between vascular beds within a single tissue in a tissue-type-dependent pattern.The EC atlas taxonomy enabled identification of EC subclusters in public scRNA-seqdatasets and provides a powerful discovery tool and resource value.
DOI: 10.1016/j.cell.2020.01.015
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30062-3