法国索邦大学Nicolas Renier和Christoph Kirst合作绘制了脑血管细尺度组织图并揭示其可塑性。2020年2月13日，国际学术期刊《细胞》在线发表了这一成果。

研究人员使用免疫标记和组织清除技术对成年小鼠大脑的血管网络进行成像，并开发了一种可对单层光显微技术获得的TB级多通道图像进行分割的方法，从而能够构建、分析和可视化由亿万个血管片段组成的血管图。研究人员利用20多个小鼠的大脑数据生成了数据集，并根据它们的解剖区域标记了动脉、静脉和毛细血管。研究人员描绘了整个脑区的血管网络组织，发现其局部适应性与功能相关。研究人员基于血管拓扑结构对皮质区域进行了分类。最后，研究人员分析了先天性耳聋和缺血性中风动物模型中脉管系统在全脑的重排，发现血管可塑性和重塑在不同模型中采用的方式不同。

研究人员表示，脑血管是动脉、静脉和毛细血管的密集网络。量化个体、脑区或疾病模型的血管组织变化具有挑战性。

附：英文原文

Title: Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature

Author: Christoph Kirst, Sophie Skriabine, Alba Vieites-Prado, Thomas Topilko, Paul Bertin, Gaspard Gerschenfeld, Florine Verny, Piotr Topilko, Nicolas Michalski, Marc Tessier-Lavigne, Nicolas Renier

Issue&Volume: February 13, 2020

Abstract: The cerebral vasculature is a dense network of arteries, capillaries, and veins. Quantifyingvariations of the vascular organization across individuals, brain regions, or diseasemodels is challenging. We used immunolabeling and tissue clearing to image the vascularnetwork of adult mouse brains and developed a pipeline to segment terabyte-sized multichannelimages from light sheet microscopy, enabling the construction, analysis, and visualizationof vascular graphs composed of over 100 million vessel segments. We generated datasetsfrom over 20 mouse brains, with labeled arteries, veins, and capillaries accordingto their anatomical regions. We characterized the organization of the vascular networkacross brain regions, highlighting local adaptations and functional correlates. Wepropose a classification of cortical regions based on the vascular topology. Finally,we analysed brain-wide rearrangements of the vasculature in animal models of congenitaldeafness and ischemic stroke, revealing that vascular plasticity and remodeling adoptdiverging rules in different models.

DOI: 10.1016/j.cell.2020.01.028

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30109-4