Title: Structural basis for strand transfer inhibitor binding to HIV intasomes
Author: Dario Oliveira Passos, Min Li, Ilona K. Jówik, Xue Zhi Zhao, Diogo Santos-Martins, Renbin Yang, Steven J. Smith, Youngmin Jeon, Stefano Forli, Stephen H. Hughes, Terrence R. Burke, Robert Craigie, Dmitry Lyumkis
Abstract: AbstractThe HIV intasome is a large nucleoprotein assembly that mediates the integration of a DNA copy of the viral genome into host chromatin. Intasomes are targeted by the latest generation of antiretrovirals, integrase (IN) strand transfer inhibitors (INSTIs). Challenges associated with lentiviral intasome biochemistry have hindered high-resolution structural studies of how INSTIs bind to their native drug target. Here, we present high-resolution cryo-electron microscopy (cryo-EM) structures of HIV intasomes bound to the latest generation INSTIs. These structures highlight how small changes in the IN active site can have significant implications for drug binding and design and provide mechanistic insights into why a leading INSTI retains efficacy against a broad spectrum of drug resistant variants. The data have implications for expanding effective treatments available for HIV-infected individuals.