美国耶鲁大学R. Todd Constable等研究人员合作实现皮质范围Ca²⁺荧光和全脑功能磁共振的同时成像。相关论文于2020年11月2日在线发表于国际学术期刊《自然—方法学》。

研究人员提出了同时宽场光学和功能磁共振成像的方法。通过合并这些模式，研究人员可以同时获取全脑血氧水平依赖性（BOLD）和全皮质钙敏感的大脑活动荧光测量值。在转基因鼠模型中，研究人员使用优化了伽玛变异传递函数的模型显示，钙可预测BOLD信号。研究人员在整个皮层中发现一致的预测，这在低频（0.009–0.08 Hz）时效果最佳。

此外，研究人员发现，模态连接强度之间的关系随区域而变化。这个方法将细胞类型的活性光学测量与最广泛使用的人脑功能评估方法联系起来。

据悉，全面对脑功能的了解需要具有互补优势的多种成像方式。

附：英文原文

Title: Simultaneous cortex-wide fluorescence Ca 2+ imaging and whole-brain fMRI

Author: Evelyn M. R. Lake, Xinxin Ge, Xilin Shen, Peter Herman, Fahmeed Hyder, Jessica A. Cardin, Michael J. Higley, Dustin Scheinost, Xenophon Papademetris, Michael C. Crair, R. Todd Constable

Issue&Volume: 2020-11-02

Abstract: Achieving a comprehensive understanding of brain function requires multiple imaging modalities with complementary strengths. We present an approach for concurrent widefield optical and functional magnetic resonance imaging. By merging these modalities, we can simultaneously acquire whole-brain blood-oxygen-level-dependent (BOLD) and whole-cortex calcium-sensitive fluorescent measures of brain activity. In a transgenic murine model, we show that calcium predicts the BOLD signal, using a model that optimizes a gamma-variant transfer function. We find consistent predictions across the cortex, which are best at low frequency (0.009–0.08 Hz). Furthermore, we show that the relationship between modality connectivity strengths varies by region. Our approach links cell-type-specific optical measurements of activity to the most widely used method for assessing human brain function. Simultaneous widefield calcium imaging and fMRI provide insight into neural activity at multiple scales and can be used to decipher the cellular origin of BOLD activity.

DOI: 10.1038/s41592-020-00984-6

Source: https://www.nature.com/articles/s41592-020-00984-6