美国威斯康星大学麦迪逊分校Joshua J. Coon、Jesse G. Meyer等研究人员合作开发出新型定量蛋白质组学分析方法。相关论文于2020年11月23日在线发表在《自然—方法学》杂志上。
Title: Quantitative shotgun proteome analysis by direct infusion
Author: Jesse G. Meyer, Natalie M. Niemi, David J. Pagliarini, Joshua J. Coon
Issue&Volume: 2020-11-23
Abstract: Liquid chromatography–mass spectrometry (LC–MS) delivers sensitive peptide analysis for proteomics but requires extensive analysis time, reducing throughput. Here, we demonstrate that gas-phase peptide separation instead of LC enables fast proteome analysis. Using direct infusion–shotgun proteome analysis (DI-SPA) by data-independent acquisition mass spectrometry (DIA-MS), we demonstrate the targeted quantification of over 500 proteins within minutes of MS data collection (~3.5 proteins per second). We show the utility of this technology in performing a complex multifactorial proteomic study of interactions between nutrients, genotype and mitochondrial toxins in a collection of cultured human cells. More than 45,000 quantitative protein measurements from 132 samples were achieved in only ~4.4h of MS data collection. Enabling fast, unbiased proteome quantification without LC, DI-SPA offers an approach to boost throughput, critical to drug and biomarker discovery studies that require analysis of thousands of proteomes.
DOI: 10.1038/s41592-020-00999-z
Source: https://www.nature.com/articles/s41592-020-00999-z
Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:28.467
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex