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科学家利用荧光报告基因成像信号网络动态
作者:小柯机器人 发布时间:2020/11/24 16:58:27

美国麻省理工学院Edward S. Boyden课题组取得一项新突破。他们的最新研究提出荧光报告基因的空间复用可用于成像信号网络动态。相关论文于2020年11月23日发表在《细胞》杂志上。

他们将荧光报告基因融合到一对自组装肽上,它可以稳定地聚集在细胞内的随机点上,其距离足以被显微镜分辨,但距离足够近,可以在空间上对相关生物学进行采样。因为可以将这些簇(他们称为信号报告岛(SiRI))进行模块化设计,所以它们可以有效地适应一组荧光报告子,以同时测量单个细胞内信号转导网络的多个节点。他们创建了用于第二信使和激酶指标的SiRI,并在培养的海马神经元和完整的脑切片中将它们用于接收cAMP驱动刺激的钙信号传导速度和PKA信号振幅之间的关系。

据介绍,为了分析信号转导网络如何将细胞输入转换为细胞输出,理想情况下,应同时测量网络内许多信号的动态。

附:英文原文

Title: Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics

Author: Changyang Linghu, Shannon L. Johnson, Pablo A. Valdes, Or A. Shemesh, Won Min Park, Demian Park, Kiryl D. Piatkevich, Asmamaw T. Wassie, Yixi Liu, Bobae An, Stephanie A. Barnes, Orhan T. Celiker, Chun-Chen Yao, Chih-Chieh (Jay) Yu, Ru Wang, Katarzyna P. Adamala, Mark F. Bear, Amy E. Keating, Edward S. Boyden

Issue&Volume: 2020-11-23

Abstract: In order to analyze how a signal transduction network converts cellular inputs into cellular outputs, ideally one would measure the dynamics of many signals within the network simultaneously. We found that, by fusing a fluorescent reporter to a pair of self-assembling peptides, it could be stably clustered within cells at random points, distant enough to be resolved by a microscope but close enough to spatially sample the relevant biology. Because such clusters, which we call signaling reporter islands (SiRIs), can be modularly designed, they permit a set of fluorescent reporters to be efficiently adapted for simultaneous measurement of multiple nodes of a signal transduction network within single cells. We created SiRIs for indicators of second messengers and kinases and used them, in hippocampal neurons in culture and intact brain slices, to discover relationships between the speed of calcium signaling, and the amplitude of PKA signaling, upon receiving a cAMP-driving stimulus.

DOI: 10.1016/j.cell.2020.10.035

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31399-4

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/