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一种通过酶促反应生成一氧化氮扩张血管的人工细胞
作者:小柯机器人 发布时间:2020/11/23 17:01:15

湖南大学刘剑波课题组与英国布里斯托大学Stephan Mann教授合作,制备了一种可以通过酶促反应生成一氧化氮从而扩张血管的人工细胞。 2020年11月20日出版的《自然—化学》发表了这项成果。

在该研究中,课题组以生物来源的凝聚层囊泡为基础,构建了一种新的人工合成的细胞模型,这种人工细胞具有优良的血液相容性和长循环时间,从而可用于递送一氧化氮产生扩张血管的作用。这种杂化原始细胞是通过在包含葡萄糖氧化酶的、由多糖-多聚核苷酸凝聚层预形成的微滴上自组装含血红蛋白的红细胞膜组分制备的。研究人员通过在空间上将葡萄糖氧化酶和血红蛋白反应串联在一起,实现了在葡萄糖和羟基脲存在的情况下在该原始细胞中源源不断地产生一氧化氮,用以探索在试管内和体内产生扩张血管的作用。

综上所述,该研究为开发和活细胞和活组织互动的、内部结构有序的细胞类似物(即生物相容性的微型机器人)提供了新的机遇。

据了解,设计和构建能与活细胞建立智能化学联系的医用人工原始细胞,是合成生物学与生物工程领域中的一个重要挑战。

附:英文原文

Title: Enzyme-mediated nitric oxide production in vasoactive erythrocyte membrane-enclosed coacervate protocells

Author: Songyang Liu, Yanwen Zhang, Mei Li, Li Xiong, Zijian Zhang, Xiaohai Yang, Xiaoxiao He, Kemin Wang, Jianbo Liu, Stephen Mann

Issue&Volume: 2020-11-20

Abstract: The design and construction of synthetic therapeutic protocells capable of establishing cognate chemical communication channels with living cells is an important challenge for synthetic biology and bio-engineering. Here we develop a step towards protocell-mediated nitric-oxide-induced vasodilation by constructing a new synthetic cell model based on bio-derived coacervate vesicles with high haemocompatibility and increased blood circulation times. The hybrid protocells are prepared by the spontaneous self-assembly of haemoglobin-containing erythrocyte membrane fragments on the surface of preformed polysaccharide–polynucleotide coacervate micro-droplets containing glucose oxidase. We use the sequestered enzymes to program a spatially coupled glucose oxidase/haemoglobin reaction cascade, which in the presence of glucose and hydroxyurea generates a protocell-mediated flux of nitric oxide that we exploit for in vitro and in vivo blood vessel vasodilation. Taken together, our results provide new opportunities for the development of endogenously organized cell-like entities (biocompatible micro-bots) geared specifically towards active interfacing with individual living cells and cell communities.

DOI: 10.1038/s41557-020-00585-y

Source: https://www.nature.com/articles/s41557-020-00585-y

期刊信息

Nature Chemistry:《自然—化学》,创刊于2009年。隶属于施普林格·自然出版集团,最新IF:21.687
官方网址:https://www.nature.com/nchem/
投稿链接:https://mts-nchem.nature.com/cgi-bin/main.plex