美国拉霍亚免疫学研究所Pandurangan Vijayanand、Christian H. Ottensmeier等研究人员合作发现,COVID-19中调节性和细胞毒性SARS-CoV-2-反应性CD4+T细胞的失衡。这一研究成果于2020年10月5日在线于《细胞》。
Title: Imbalance of regulatory and cytotoxic SARS-CoV-2-reactive CD4+ T cells in COVID-19
Author: Benjamin J. Meckiff, Ciro Ramírez-Suástegui, Vicente Fajardo, Serena J. Chee, Anthony Kusnadi, Hayley Simon, Simon Eschweiler, Alba Grifoni, Emanuela Pelosi, Daniela Weiskopf, Alessandro Sette, Ferhat Ay, Grégory Seumois, Christian H. Ottensmeier, Pandurangan Vijayanand
Issue&Volume: 2020-10-05
Abstract: The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper (TFH) cells and cytotoxic T helper cells (CD4-CTLs) responding to SARS-CoV-2, and reduced proportion of SARS-CoV-2-reactive regulatory T cells (TREG). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic TFH response was observed early in the illness which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional T helper (TH)1 and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+ T cells compared to influenza-reactive CD4+ T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4+ T cells in distinct disease severities.
DOI: 10.1016/j.cell.2020.10.001
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31307-6