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COVID-19中调节性和细胞毒性SARS-CoV-2-反应性CD4+T细胞出现失衡
作者:小柯机器人 发布时间:2020/10/9 16:32:21

美国拉霍亚免疫学研究所Pandurangan Vijayanand、Christian H. Ottensmeier等研究人员合作发现,COVID-19中调节性和细胞毒性SARS-CoV-2-反应性CD4+T细胞的失衡。这一研究成果于2020年10月5日在线于《细胞》。

研究人员对来自40个COVID-19患者的超过100000个病毒抗原反应性CD4+T细胞进行了单细胞转录组学分析。与非住院患者相比,住院患者中发现对SARS-CoV-2有反应的细胞毒性滤泡辅助细胞(TFH)和细胞毒性T辅助细胞(CD4-CTL)的比例增加,而SARS-CoV-2-的比例降低反应性调节性T细胞(TREG)。
 
重要的是,在住院的COVID-19患者中,在疾病早期观察到了强烈的细胞毒性TFH反应,这与SARS-CoV-2突刺蛋白的抗体水平呈负相关。与流感反应性CD4+T细胞相比,SARS-CoV-2-反应性CD4+T细胞库中多功能T辅助(TH)1和TH17细胞亚群不足。因此,这些分析提供了不同疾病严重程度下SARS-CoV-2-反应性CD4+T细胞基因表达模式的见解。
 
据悉,CD4+T细胞对SARS-CoV-2感染的保护性或病原性免疫反应的贡献仍然未知。
 
附:英文原文

Title: Imbalance of regulatory and cytotoxic SARS-CoV-2-reactive CD4+ T cells in COVID-19

Author: Benjamin J. Meckiff, Ciro Ramírez-Suástegui, Vicente Fajardo, Serena J. Chee, Anthony Kusnadi, Hayley Simon, Simon Eschweiler, Alba Grifoni, Emanuela Pelosi, Daniela Weiskopf, Alessandro Sette, Ferhat Ay, Grégory Seumois, Christian H. Ottensmeier, Pandurangan Vijayanand

Issue&Volume: 2020-10-05

Abstract: The contribution of CD4+ T cells to protective or pathogenic immune responses to SARS-CoV-2 infection remains unknown. Here, we present single-cell transcriptomic analysis of >100,000 viral antigen-reactive CD4+ T cells from 40 COVID-19 patients. In hospitalized patients compared to non-hospitalized patients, we found increased proportions of cytotoxic follicular helper (TFH) cells and cytotoxic T helper cells (CD4-CTLs) responding to SARS-CoV-2, and reduced proportion of SARS-CoV-2-reactive regulatory T cells (TREG). Importantly, in hospitalized COVID-19 patients, a strong cytotoxic TFH response was observed early in the illness which correlated negatively with antibody levels to SARS-CoV-2 spike protein. Polyfunctional T helper (TH)1 and TH17 cell subsets were underrepresented in the repertoire of SARS-CoV-2-reactive CD4+ T cells compared to influenza-reactive CD4+ T cells. Together, our analyses provide insights into the gene expression patterns of SARS-CoV-2-reactive CD4+ T cells in distinct disease severities.

DOI: 10.1016/j.cell.2020.10.001

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31307-6

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/