近日，美国斯克里普斯研究所Kirill A. Martemyanov团队绘制出RGS蛋白调控G蛋白信号的全局图谱。相关论文于2020年10月1日在线发表于国际学术期刊《细胞》。

Title: A Global Map of G Protein Signaling Regulation by RGS Proteins

Author: Ikuo Masuho, Santhanam Balaji, Brian S. Muntean, Nickolas K. Skamangas, Sreenivas Chavali, John J.G. Tesmer, M. Madan Babu, Kirill A. Martemyanov

Issue&Volume: 2020-10-1

Abstract: The control over the extent and timing of G protein signaling is provided by the regulatorof G protein signaling (RGS) proteins that deactivate G protein α subunits (Gα). Mammaliangenomes encode 20 canonical RGS and 16 Gα genes with key roles in physiology and disease.To understand the principles governing the selectivity of Gα regulation by RGS, weexamine the catalytic activity of all canonical human RGS proteins and their selectivityfor a complete set of Gα substrates using real-time kinetic measurements in livingcells. The data reveal rules governing RGS-Gα recognition, the structural basis ofits selectivity, and provide principles for engineering RGS proteins with definedselectivity. The study also explores the evolution of RGS-Gα selectivity through ancestralreconstruction and demonstrates how naturally occurring non-synonymous variants inRGS alter signaling. These results provide a blueprint for decoding signaling selectivityand advance our understanding of molecular recognition principles.

DOI: 10.1016/j.cell.2020.08.052

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31140-5