美国加州理工学院Mitchell Guttman、佛蒙特大学Devdoot Majumdar等研究人员合作发现，SARS-CoV-2破坏剪接、翻译和蛋白质运输以抑制宿主防御。相关论文于2020年10月8日在线发表于国际学术期刊《细胞》。

Title: SARS-CoV-2 disrupts splicing, translation, and protein trafficking to suppress host defenses

Author: Abhik K. Banerjee, Mario R. Blanco, Emily A. Bruce, Drew D. Honson, Linlin M. Chen, Amy Chow, Prashant Bhat, Noah Ollikainen, Sofia A. Quinodoz, Colin Loney, Jasmine Thai, Zachary D. Miller, Aaron E. Lin, Madaline M. Schmidt, Douglas G. Stewart, Daniel Goldfarb, Giuditta De Lorenzo, Suzannah J. Rihn, Rebecca Voorhees, Jason W. Botten, Devdoot Majumdar, Mitchell Guttman

Issue&Volume: 2020-10-08

Abstract: SARS-CoV-2 is a recently identified coronavirus that causes the respiratory disease known as COVID-19. Despite the urgent need, we still do not fully understand the molecular basis of SARS-CoV-2 pathogenesis. Here, we comprehensively define the interactions between SARS-CoV-2 proteins and human RNAs. NSP16 binds to the mRNA recognition domains of the U1 and U2 splicing RNAs and acts to suppress global mRNA splicing upon SARS-CoV-2 infection. NSP1 binds to 18S ribosomal RNA in the mRNA entry channel of the ribosome and leads to global inhibition of mRNA translation upon infection. Finally, NSP8 and NSP9 bind to the 7SL RNA in the Signal Recognition Particle and interfere with protein trafficking to the cell membrane upon infection. Disruption of each of these essential cellular functions acts to suppress the interferon response to viral infection. Our results uncover a multipronged strategy utilized by SARS-CoV-2 to antagonize essential cellular processes to suppress host defenses.

DOI: 10.1016/j.cell.2020.10.004

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31310-6