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科学家开发出新的亚细胞水平成像技术
作者:小柯机器人 发布时间:2020/1/7 15:40:14

美国耶鲁医学院Joerg Bewersdorf课题组利用多色三维荧光成像技术揭示了纳米级亚细胞结构。这一研究成果在线发表在2020年1月6日的《自然—方法学》上。

研究介绍了一种4Pi单分子转换超分辨率显微镜,该显微镜可以使用“挽救荧光”在3维上以5-10 nm的定位精度对哺乳动物细胞进行比例多色成像。他们表示,同时成像两个或三个荧光团解决了高度扭曲的高尔基体以及内质网和质膜之间紧密接触的荧光成像问题,传统上这些结构只能使用电镜成像。挽救荧光方法同样适用于大多数单物镜显微镜。

据悉,将荧光探针的分子特异性与纳米分辨率的三维成像相结合对于研究细胞器和蛋白质复合物的空间组织和相互作用至关重要。

附:英文原文

Title: Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging

Author: Yongdeng Zhang, Lena K. Schroeder, Mark D. Lessard, Phylicia Kidd, Jeeyun Chung, Yuanbin Song, Lorena Benedetti, Yiming Li, Jonas Ries, Jonathan B. Grimm, Luke D. Lavis, Pietro De Camilli, James E. Rothman, David Baddeley, Joerg Bewersdorf

Issue&Volume: 2020-01-06

Abstract: Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5–10-nm localization precision in three dimensions using ‘salvaged fluorescence’. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.

DOI: 10.1038/s41592-019-0676-4

Source: https://www.nature.com/articles/s41592-019-0676-4

期刊信息

Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:28.467
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex