美国哥伦比亚大学生物医学工程系Elizabeth M. C. Hillman研究组，使用SCAPE 2.0平台对体内动力学和大规模样品进行实时显微镜成像，相关论文发表在2019年10月刊的《自然—方法学》上。

研究人员将高速增强型摄像头技术与功能广泛、可重新配置且显著改进的后置共聚焦对准平面激发（SCAPE）显微镜设计相结合，可以实现每秒300多体量和1.2 GHz像素速率的高分辨率体积成像。

研究人员展示了自由运动的秀丽隐杆线虫的近等向采样，并分析了跳动的斑马鱼心脏中的实时血流量和钙动力学。该系统还允许对浸没的、完整的、清除的和扩展的样品进行高通量结构成像。与点扫描技术相比，SCAPE 2.0的光损伤明显更低。

这些结果表明，SCAPE 2.0是功能强大且易使用的成像平台，适用于各种新兴的高速动态和高通量体积显微镜应用。

研究人员介绍，大多数显微镜方法有限的像素带宽需要在视场、采样密度和成像速度之间做出折衷。这种局限性限制了涉及复杂运动或快速细胞信号传导的研究，凸显了高通量结构成像的主要瓶颈。

附：英文原文

Title: Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0

Author: Venkatakaushik Voleti, Kripa B. Patel, Wenze Li, Citlali Perez Campos, Srinidhi Bharadwaj, Hang Yu, Caitlin Ford, Malte J. Casper, Richard Wenwei Yan, Wenxuan Liang, Chentao Wen, Koutarou D. Kimura, Kimara L. Targoff & Elizabeth M. C. Hillman 

Issue&Volume: Volume 16 Issue 10

Abstract:

The limited per-pixel bandwidth of most microscopy methods requires compromises between field of view, sampling density and imaging speed. This limitation constrains studies involving complex motion or fast cellular signaling, and presents a major bottleneck for high-throughput structural imaging. Here, we combine high-speed intensified camera technology with a versatile, reconfigurable and dramatically improved Swept, Confocally Aligned Planar Excitation (SCAPE) microscope design that can achieve high-resolution volumetric imaging at over 300 volumes per second and over 1.2 GHz pixel rates. We demonstrate near-isotropic sampling in freely moving Caenorhabditis elegans, and analyze real-time blood flow and calcium dynamics in the beating zebrafish heart. The same system also permits high-throughput structural imaging of mounted, intact, cleared and expanded samples. SCAPE 2.0’s significantly lower photodamage compared to point-scanning techniques is also confirmed. Our results demonstrate that SCAPE 2.0 is a powerful, yet accessible imaging platform for myriad emerging high-speed dynamic and high-throughput volumetric microscopy applications.

DOI: 10.1038/s41592-019-0579-4

Source: https://www.nature.com/articles/s41592-019-0579-4