福建农林大学明瑞光（Ray Ming）、奥地利维也纳大学Christian Lexer和美国乔治亚大学Jeffrey L. Bennetzen等研究人员合作绘制菠萝驯化基因组图谱。该研究于2019年9月30日在线发表于《自然—遗传学》。

对来自南美的无性繁殖作物（例如菠萝）的驯化被认为是“一步操作”。研究人员对菠萝（Ananas comosus var. bracteatus CB5）进行了测序，将513 Mb测序数据组装成具有29412个基因的25条染色体。CB5、F153和MD2基因组的比较阐明了纤维产生、颜色形成、糖分积累和果实成熟的基因组基础。

研究人员还对89个菠萝基因组进行了重新测序。“ Smooth Cayenne”和“ Queen”品种表现出古老的和最近的混合物种，而“ Singapore Spanish”则支持一步一步的驯化操作。研究人员确定了25次选择性筛除，包括一次含有一对串联重复的菠萝蛋白酶抑制基因的强烈筛除。四个自不相容候选基因在F153中发现，但在自相容的CB5中不起作用。

这些发现表明在无性繁殖农作物的驯化中有性重组和一步操作的共存。这项工作为其他无性繁殖作物中有性和无性驯化轨迹的探索提供了指导。

附：英文原文

Title: The bracteatus pineapple genome and domestication of clonally propagated crops

Author: Li-Yu Chen, Robert VanBuren, Margot Paris, Hongye Zhou, Xingtan Zhang, Ching Man Wai, Hansong Yan, Shuai Chen, Michael Alonge, Srividya Ramakrishnan, Zhenyang Liao, Juan Liu, Jishan Lin, Jingjing Yue, Mahpara Fatima, Zhicong Lin, Jisen Zhang, Lixian Huang, Hao Wang, Teh-Yang Hwa, Shu-Min Kao, Jae Young Choi, Anupma Sharma, Jian Song, Lulu Wang, Won C. Yim, John C. Cushman, Robert E. Paull, Tracie Matsumoto, Yuan Qin, Qingsong Wu, Jianping Wang, Qingyi Yu, Jun Wu, Shaoling Zhang, Peter Boches, Chih-Wei Tung, Ming-Li Wang, Geo Coppens dEeckenbrugge, Garth M. Sanewski, Michael D. Purugganan, Michael C. Schatz, Jeffrey L. Bennetzen, Christian Lexer, Ray Ming

Issue&Volume: 2019-09-30

Abstract: 

Domestication of clonally propagated crops such as pineapple from South America was hypothesized to be a ‘one-step operation’. We sequenced the genome of Ananas comosus var. bracteatus CB5 and assembled 513 Mb into 25 chromosomes with 29,412 genes. Comparison of the genomes of CB5, F153 and MD2 elucidated the genomic basis of fiber production, color formation, sugar accumulation and fruit maturation. We also resequenced 89 Ananas genomes. Cultivars ‘Smooth Cayenne’ and ‘Queen’ exhibited ancient and recent admixture, while ‘Singapore Spanish’ supported a one-step operation of domestication. We identified 25 selective sweeps, including a strong sweep containing a pair of tandemly duplicated bromelain inhibitors. Four candidate genes for self-incompatibility were linked in F153, but were not functional in self-compatible CB5. Our findings support the coexistence of sexual recombination and a one-step operation in the domestication of clonally propagated crops. This work guides the exploration of sexual and asexual domestication trajectories in other clonally propagated crops.

DOI: 10.1038/s41588-019-0506-8

Source:https://www.nature.com/articles/s41588-019-0506-8