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科学家分离出有效的新冠病毒中和抗体
作者:小柯机器人 发布时间:2020/6/17 20:52:38

美国斯克里普斯研究所的Dennis R. Burton、Joseph G. Jardine、Devin Sok等研究人员合作分离出有效的新冠病毒(SARS-CoV-2)中和抗体,可在小动物模型中抵御新冠感染。2020年6月15日,《科学》杂志在线发表了这一研究成果。

研究人员招募了一组SARS-CoV-2康复参与者,开发了中和测定法来研究抗体反应,调整了高通量抗体生成流程以快速筛选1800多种抗体,并建立了动物模型来测试保护性。研究人员分离了针对受体结合结构域(RBD)上两个表位以及对突刺(S)蛋白上不同的非RBD表位的有效中和抗体(nAb)。
 
研究人员显示,nAb的被动转移可为叙利亚仓鼠在大剂量SARS-CoV-2攻击中抵抗疾病提供保护,在被治疗动物中体重得以维持和肺病毒滴度较低。
 
该研究表明,nAb在预防和潜在治疗COVID-19中发挥作用。此外,nAb也定义了保护性表位,可用于指导疫苗设计。
 
附:英文原文

Title: Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model

Author: Thomas F. Rogers, Fangzhu Zhao, Deli Huang, Nathan Beutler, Alison Burns, Wan-ting He, Oliver Limbo, Chloe Smith, Ge Song, Jordan Woehl, Linlin Yang, Robert K. Abbott, Sean Callaghan, Elijah Garcia, Jonathan Hurtado, Mara Parren, Linghang Peng, Sydney Ramirez, James Ricketts, Michael J. Ricciardi, Stephen A. Rawlings, Nicholas C. Wu, Meng Yuan, Davey M. Smith, David Nemazee, John R. Teijaro, James E. Voss, Ian A. Wilson, Raiees Andrabi, Bryan Briney, Elise Landais, Devin Sok, Joseph G. Jardine, Dennis R. Burton

Issue&Volume: 2020/06/15

Abstract: Abstract Countermeasures to prevent and treat COVID-19 are a global health priority. We enrolled a cohort of SARS-CoV-2-recovered participants, developed neutralization assays to interrogate antibody responses, adapted our high-throughput antibody generation pipeline to rapidly screen over 1800 antibodies, and established an animal model to test protection. We isolated potent neutralizing antibodies (nAbs) to two epitopes on the receptor binding domain (RBD) and to distinct non-RBD epitopes on the spike (S) protein. We showed that passive transfer of a nAb provides protection against disease in high-dose SARS-CoV-2 challenge in Syrian hamsters, as revealed by maintained weight and low lung viral titers in treated animals. The study suggests a role for nAbs in prophylaxis, and potentially therapy, of COVID-19. The nAbs define protective epitopes to guide vaccine design.

DOI: 10.1126/science.abc7520

Source: https://science.sciencemag.org/content/early/2020/06/15/science.abc7520

期刊信息
Science:《科学》,创刊于1880年。隶属于美国科学促进会,最新IF:41.037