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科学家建立非洲绿猴COVID-19感染模型
作者:小柯机器人 发布时间:2020/11/26 11:01:20

美国德克萨斯大学Thomas W. Geisbert小组建立了非洲绿猴COVID-19感染模型。相关论文于2020年11月24日在线发表在《自然—免疫学》杂志上。

研究人员发现,非洲绿猴(AGM)能够实现SARS-CoV-2的显著复制并产生明显的呼吸系统疾病,这可能比其他非人类灵长类动物更准确地反映出人类COVID-19病例。暴露后15天,在包括直肠拭子在内的粘膜样品中检测到SARS-CoV-2。血液和组织中明显的炎症和凝血病是突出特征。转录组分析表明,支气管肺泡灌洗样品中的干扰素和白介素6通路激活,并抑制了外周血中与自然杀伤细胞和T细胞相关的转录本。
 
尽管初次感染后抗体效价略有下降,但增强的抗体和细胞反应有助于在用相同毒株再次感染后的迅速清除。这些数据支持AGM在研究COVID-19发病机理和测试医疗方案方面的实用性。
 
据了解,SARS-CoV-2导致了全球COVID-19的空前流行。目前迫切需要动物模型来研究COVID-19的发病机理并筛选疫苗和治疗方法。
 
附:英文原文

Title: Establishment of an African green monkey model for COVID-19 and protection against re-infection

Author: Courtney Woolsey, Viktoriya Borisevich, Abhishek N. Prasad, Krystle N. Agans, Daniel J. Deer, Natalie S. Dobias, John C. Heymann, Stephanie L. Foster, Corri B. Levine, Liana Medina, Kevin Melody, Joan B. Geisbert, Karla A. Fenton, Thomas W. Geisbert, Robert W. Cross

Issue&Volume: 2020-11-24

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for an unprecedented global pandemic of COVID-19. Animal models are urgently needed to study the pathogenesis of COVID-19 and to screen vaccines and treatments. We show that African green monkeys (AGMs) support robust SARS-CoV-2 replication and develop pronounced respiratory disease, which may more accurately reflect human COVID-19 cases than other nonhuman primate species. SARS-CoV-2 was detected in mucosal samples, including rectal swabs, as late as 15 days after exposure. Marked inflammation and coagulopathy in blood and tissues were prominent features. Transcriptome analysis demonstrated stimulation of interferon and interleukin-6 pathways in bronchoalveolar lavage samples and repression of natural killer cell- and T cell–associated transcripts in peripheral blood. Despite a slight waning in antibody titers after primary challenge, enhanced antibody and cellular responses contributed to rapid clearance after re-challenge with an identical strain. These data support the utility of AGM for studying COVID-19 pathogenesis and testing medical countermeasures.

DOI: 10.1038/s41590-020-00835-8

Source: https://www.nature.com/articles/s41590-020-00835-8

期刊信息

Nature Immunology:《自然—免疫学》,创刊于2000年。隶属于施普林格·自然出版集团,最新IF:23.53
官方网址:https://www.nature.com/ni/
投稿链接:https://mts-ni.nature.com/cgi-bin/main.plex