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新方法可研究单RNA病毒的翻译和复制动态
作者:小柯机器人 发布时间:2020/11/16 15:24:48

荷兰乌得勒支大学医学中心Marvin E. Tanenbaum、Frank J.M. van Kuppeveld等研究人员合作,开发出研究单RNA病毒翻译和复制动态的新方法。相关论文于2020年11月13日在线发表于《细胞》。

研究人员开发了一种单分子成像测定法,即病毒感染实时成像(VIRIM),能够研究活细胞中单个RNA病毒的翻译和复制。VIRIM在细胞之间的复制动力学中发现了惊人的异质性,并揭示了单个病毒RNA的翻译和复制之间的广泛协调。
 
此外,使用VIRIM,研究人员将传入病毒RNA的复制步骤确定为成功感染的主要瓶颈,并确定了负责抑制早期病毒复制的宿主基因。病毒感染的单分子成像是研究病毒复制和病毒-宿主相互作用的强大工具,可广泛应用于RNA病毒。
 
研究人员介绍,RNA病毒是最普遍的病原体之一,也是社会的主要负担。尽管已经对RNA病毒进行了广泛的研究,但由于缺乏灵敏的检测方法,因此对于感染最初几个小时内的发生过程知之甚少。
 
附:英文原文

Title: Translation and Replication Dynamics of Single RNA Viruses

Author: Sanne Boersma, Huib H. Rabouw, Lucas J.M. Bruurs, Tonja Pavlovi, Arno L.W. van Vliet, Joep Beumer, Hans Clevers, Frank J.M. van Kuppeveld, Marvin E. Tanenbaum

Issue&Volume: 2020-11-13

Abstract: RNA viruses are among the most prevalent pathogens and are a major burden on society. Although RNA viruses have been studied extensively, little is known about the processes that occur during the first several hours of infection because of a lack of sensitive assays. Here we develop a single-molecule imaging assay, virus infection real-time imaging (VIRIM), to study translation and replication of individual RNA viruses in live cells. VIRIM uncovered a striking heterogeneity in replication dynamics between cells and revealed extensive coordination between translation and replication of single viral RNAs. Furthermore, using VIRIM, we identify the replication step of the incoming viral RNA as a major bottleneck of successful infection and identify host genes that are responsible for inhibition of early virus replication. Single-molecule imaging of virus infection is a powerful tool to study virus replication and virus-host interactions that may be broadly applicable to RNA viruses.

DOI: 10.1016/j.cell.2020.10.019

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)31382-9

期刊信息
Cell:《细胞》,创刊于1974年。隶属于细胞出版社,最新IF:36.216
官方网址:https://www.cell.com/