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癫痫小鼠中存在空间编码和神经元间同步性的破坏
作者:小柯机器人 发布时间:2020/1/9 14:43:46

美国加州大学洛杉矶分校Peyman Golshani、希腊分子生物学与生物技术实验室Panayiota Poirazi、美国西奈山伊坎医学院Tristan Shuman等研究人员合作发现,癫痫小鼠中存在空间编码和神经元间同步性的破坏。该项研究成果于2020年1月6日在线发表于《自然—神经科学》杂志。

研究人员表示,颞叶癫痫会导致严重的认知功能障碍,但其回路机制仍不清楚。癫痫发生过程中神经元间的死亡和重组可能破坏海马抑制的同步性。

为了测试这一点,研究人员在头戴式虚拟导航仪中用硅探针从毛果芸香碱治疗的癫痫小鼠中同时记录了的CA1区和齿状回。研究人员发现癫痫小鼠CA1和齿状回之间的不同步神经元间信号。由于海马中间神经元控制信息处理,研究人员使用新型的无线微型镜测试了CA1空间编码在该去同步回路中是否发生了变化。研究人员发现癫痫小鼠中的CA1位置细胞不稳定,并在一周内完全重新映射。这种空间的不稳定性在癫痫持续状态后约6周出现,也就是在慢性癫痫发作和神经元间死亡开始后很久。最后,CA1网络建模表明,不同步的输入会损害CA1位置单元的精度和稳定性。总之,这些结果表明,时间精确的海马内通信对于空间处理至关重要。

附:英文原文

Title: Breakdown of spatial coding and interneuron synchronization in epileptic mice

Author: Tristan Shuman, Daniel Aharoni, Denise J. Cai, Christopher R. Lee, Spyridon Chavlis, Lucia Page-Harley, Lauren M. Vetere, Yu Feng, Chen Yi Yang, Irene Mollinedo-Gajate, Lingxuan Chen, Zachary T. Pennington, Jiannis Taxidis, Sergio E. Flores, Kevin Cheng, Milad Javaherian, Christina C. Kaba, Naina Rao, Mimi La-Vu, Ioanna Pandi, Matthew Shtrahman, Konstantin I. Bakhurin, Sotiris C. Masmanidis, Baljit S. Khakh, Panayiota Poirazi, Alcino J. Silva, Peyman Golshani

Issue&Volume: 2020-01-06

Abstract: Temporal lobe epilepsy causes severe cognitive deficits, but the circuit mechanisms remain unknown. Interneuron death and reorganization during epileptogenesis may disrupt the synchrony of hippocampal inhibition. To test this, we simultaneously recorded from the CA1 and dentate gyrus in pilocarpine-treated epileptic mice with silicon probes during head-fixed virtual navigation. We found desynchronized interneuron firing between the CA1 and dentate gyrus in epileptic mice. Since hippocampal interneurons control information processing, we tested whether CA1 spatial coding was altered in this desynchronized circuit, using a novel wire-free miniscope. We found that CA1 place cells in epileptic mice were unstable and completely remapped across a week. This spatial instability emerged around 6 weeks after status epilepticus, well after the onset of chronic seizures and interneuron death. Finally, CA1 network modeling showed that desynchronized inputs can impair the precision and stability of CA1 place cells. Together, these results demonstrate that temporally precise intrahippocampal communication is critical for spatial processing.

DOI: 10.1038/s41593-019-0559-0

Source: https://www.nature.com/articles/s41593-019-0559-0

期刊信息

Nature Neuroscience:《自然—神经科学》,创刊于1998年。隶属于施普林格·自然出版集团,最新if:21.126
官方网址:https://www.nature.com/neuro/
投稿链接:https://mts-nn.nature.com/cgi-bin/main.plex