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研究绘制病毒和致癌物引起的头颈癌免疫图谱
作者:小柯机器人 发布时间:2020/1/8 11:11:43

近日,美国匹兹堡大学医学院Dario A.A. Vignali、Robert L. Ferris、Tullia C. Bruno等研究人员合作描绘了病毒和致癌物引起的头颈癌免疫图谱。该项研究成果2020年1月7日在线发表在《免疫》上。

研究人员表示,头颈部鳞状细胞癌(HNSCC)是由于暴露于环境致癌物或人乳头瘤病毒(HPV)引起的恶性转化而引起的。

研究人员评估了HPV和HPV+HNSCC患者以及健康供体的外周和肿瘤内免疫群体中131,224个单细胞的转录谱。HPV和HPV+HNSCC肿瘤内的免疫细胞表现出一系列转录特征,CD4+辅助T细胞和B细胞相对不同,而CD8+T细胞和CD4+调节性T细胞相对类似。转录结果通过多光谱免疫荧光分析和基于空间邻近性的潜在细胞与细胞交流来进行分析。这些分析定义了与CD4+滤泡辅助性T细胞相关的基因表达特征,其与HNSCC患者更长的无进展生存期相关。这些数据集和分析方法为进一步研究免疫细胞对病毒和致癌物诱发癌症的影响提供了资源。

附:英文原文

Title: Immune Landscape of Viral- and Carcinogen-Driven Head and Neck Cancer

Author: Anthony R. Cillo, Cornelius H.L. Kürten, Tracy Tabib, Zengbiao Qi, Sayali Onkar, Ting Wang, Angen Liu, Umamaheswar Duvvuri, Seungwon Kim, Ryan J. Soose, Steffi Oesterreich, Wei Chen, Robert Lafyatis, Tullia C. Bruno, Robert L. Ferris, Dario A.A. Vignali

Issue&Volume: January 7, 2020

Abstract: Head and neck squamous cell carcinoma (HNSCC) arises through exposure to environmentalcarcinogens or malignant transformation by human papillomavirus (HPV). Here, we assessedthe transcriptional profiles of 131,224 single cells from peripheral and intra-tumoralimmune populations from patients with HPV– and HPV+ HNSCC and healthy donors. Immune cells within tumors of HPV– and HPV+ HNSCC displayed a spectrum of transcriptional signatures, with helper CD4+ T cells and B cells being relatively divergent and CD8+ T cells and CD4+ regulatoryT cells being relatively similar. Transcriptional results were contextualized throughmultispectral immunofluorescence analyses and evaluating putative cell-cell communicationbased on spatial proximity. These analyses defined a gene expression signature associatedwith CD4+ T follicular helper cells that is associated with longer progression-free survivalin HNSCC patients. The datasets and analytical approaches herein provide a resourcefor the further study of the impact of immune cells on viral- and carcinogen-inducedcancers.

DOI: 10.1016/j.immuni.2019.11.014

Source: https://www.cell.com/immunity/fulltext/S1074-7613(19)30495-9

期刊信息

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新if:21.522
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx