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普通菜豆最大规模重测序和表型精准鉴定
作者:小柯机器人 发布时间:2019/12/24 12:03:44

近日,中国农业科学院作物科学研究所王述民团队与美国田纳西州立大学Matthew Blair教授合作对683种常见豆基因型进行重测序,从而确定了南北跨系的产量构成特征性状关联。2019年12月23日,《自然—遗传学》在线发表了这项成果。

研究人员进行了大规模的全基因组关联研究,评估了3年以上、在中国四个纬度为18.23°至45.75°N、种植日期不同的683个常见豆种,包括地方品种和育种系,并存在非生物或生物压力。总共505个位点与产量构成因素相关,其中种子大小、开花时间和收获成熟性状在多年和环境中稳定。一些基因座与控制这些性状的候选基因相关。研究人员观察到产量组成部分与第1号染色体长臂上的一个富含基因的区域有很强的联系。通过选择种子长度与种子宽度和高度来改变种子大小是可能的,这提供了一种基于基因组的手段来影响重要的产量组成因素。这项研究表明,评估南北地理区别的大型种质资源有助于检测标志物关联,从而确定豆类的产量。

附:英文原文

Title: Resequencing of 683 common bean genotypes identifies yield component trait associations across a north–south cline

Author: Jing Wu, Lanfen Wang, Junjie Fu, Jibao Chen, Shuhong Wei, Shilong Zhang, Jie Zhang, Yongsheng Tang, Mingli Chen, Jifeng Zhu, Lei Lei, Qinghe Geng, Chunliang Liu, Lei Wu, Xiaoming Li, Xiaoli Wang, Qiang Wang, Zhaoli Wang, Shilai Xing, Haikuan Zhang, Matthew W. Blair, Shumin Wang

Issue&Volume: 2019-12-23

Abstract: We conducted a large-scale genome-wide association study evaluation of 683common bean accessions, including landraces and breeding lines, grown over 3years and in four environments across China, ranging in latitude from 18.23° to 45.75° N, with different planting dates and abiotic or biotic stresses. A total of 505loci were associated with yield components, of which seed size, flowering time and harvest maturity traits were stable across years and environments. Some loci aligned with candidate genes controlling these traits. Yield components were observed to have strong associations with a gene-rich region on the long arm of chromosome1. Manipulation of seed size, through selection of seed length versus seed width and height, was deemed possible, providing a genome-based means to select for important yield components. This study shows that evaluation of large germplasm collections across north–south geographic clines is useful in the detection of marker associations that determine grain yield in pulses.

DOI: 10.1038/s41588-019-0546-0

Source: https://www.nature.com/articles/s41588-019-0546-0

期刊信息

Nature Genetics:《自然—遗传学》,创刊于1992年。隶属于施普林格·自然出版集团,最新IF:25.455
官方网址:https://www.nature.com/ng/
投稿链接:https://mts-ng.nature.com/cgi-bin/main.plex