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通过分生组织从头诱导可实现植物基因编辑
作者:小柯机器人 发布时间:2019/12/18 13:24:40

美国明尼苏达大学Daniel F. Voytas课题组近日取得一项新成果。他们开发出通过出分生组织从头诱导的植物基因编辑技术。2019年12月16日,国际知名学术期刊《自然—生物技术》在线发表了这一成果。

研究人员报告了两种通过从头分生组织诱导产生基因编辑的双子叶植物的方法。发育调节因子和基因编辑试剂被输送到整个植物的体细胞中。这会诱导分生组织产生具有目标DNA修饰的芽,并将基因编辑传递给下一代。从头诱导基因编辑的分生组织回避了组织培养的需要,并有望克服植物基因编辑的瓶颈。

据了解,植物基因编辑通常是通过向培养物中的外植体递送诸如Cas9和单向导RNA的试剂来进行。 然后通过暴露于各种激素来诱导编辑后的细胞分化为完整植物。通过组织培养创造经过编辑的植物通常效率低下、费时,仅适用于有限的物种和基因型,并且会导致基因组和表观基因组发生意外变化。

附:英文原文

Title: Plant gene editing through de novo induction of meristems

Author: Michael F. Maher, Ryan A. Nasti, Macy Vollbrecht, Colby G. Starker, Matthew D. Clark, Daniel F. Voytas

Issue&Volume: 2019-12-16

Abstract: Plant gene editing is typically performed by delivering reagents such as Cas9 and single guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole plants by exposure to various hormones. The creation of edited plants through tissue culture is often inefficient, time-consuming, works for only limited species and genotypes, and causes unintended changes to the genome and epigenome. Here we report two methods to generate gene-edited dicotyledonous plants through de novo meristem induction. Developmental regulators and gene-editing reagents are delivered to somatic cells of whole plants. This induces meristems that produce shoots with targeted DNA modifications, and gene edits are transmitted to the next generation. The de novo induction of gene-edited meristems sidesteps the need for tissue culture and promises to overcome a bottleneck in plant gene editing.

DOI: 10.1038/s41587-019-0337-2

Source: https://www.nature.com/articles/s41587-019-0337-2

期刊信息

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:31.864
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex