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过硫化物检测揭示其抗衰老作用
作者:小柯机器人 发布时间:2019/11/15 14:56:05

法国波尔多大学Milos R. Filipovic研究组发现,选择性过硫化物检测揭示过硫化具有保守的抗衰老作用。相关论文2019年11月14日发表在国际学术期刊《细胞—代谢》上。

研究使用二甲酮类探针进行化学选择性过硫生物偶联的简便策略,在具有广泛用途的生物样品中实现高选择性、快速和稳健的过硫标记。使用这种方法,研究表明,过硫化是一种进化上保守的修饰,过硫化波被细胞用来解决亚磺酰基化并防止不可逆的半胱氨酸过氧化,从而保留了蛋白质的功能。他们报道了与衰老相关的过硫化反应的下降,这在进化边界上是保守的。因此,增加过硫化作用的饮食或药理干预措施会增加寿命,并增加应对压力刺激的能力。

研究人员表示,地球上的生命早已出现在富含硫化氢(H2S)的环境中,并且由H2S介导的蛋白质过硫化进化为一种信号传导机制。蛋白质过硫化(S-巯基化)是反应性半胱氨酸残基的翻译后修饰,可调节蛋白质的结构和/或功能。由于过硫化物与半胱氨酸的反应性和相似性,因此难以标记和研究。

附:英文原文

Title: Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration

Author: Jasmina Zivanovic, Emilia Kouroussis, Joshua B. Kohl, Bikash Adhikari, Biljana Bursac, Sonia Schott-Roux, Dunja Petrovic, Jan Lj. Miljkovic, Daniel Thomas-Lopez, Youngeun Jung, Marko Miler, Sarah Mitchell, Verica Milosevic, Jose Eduardo Gomes, Moran Benhar, Bruno Gonzales-Zorn, Ivana Ivanovic-Burmazovic, Roberta Torregrossa, James R. Mitchell, Matthew Whiteman, Guenter Schwarz, Solomon H. Snyder, Bindu D. Paul, Kate S. Carroll, Milos R. Filipovic

Issue&Volume: November 14, 2019

Abstract: Life on Earth emerged in a hydrogen sulfide (H2S)-rich environment eons ago and with it protein persulfidation mediated by H2S evolved as a signaling mechanism. Protein persulfidation (S-sulfhydration) is a post-translational modification of reactive cysteine residues,which modulate protein structure and/or function. Persulfides are difficult to labeland study due to their reactivity and similarity with cysteine. Here, we report afacile strategy for chemoselective persulfide bioconjugation using dimedone-basedprobes, to achieve highly selective, rapid, and robust persulfide labeling in biologicalsamples with broad utility. Using this method, we show persulfidation is an evolutionarilyconserved modification and waves of persulfidation are employed by cells to resolvesulfenylation and prevent irreversible cysteine overoxidation preserving protein function.We report an age-associated decline in persulfidation that is conserved across evolutionaryboundaries. Accordingly, dietary or pharmacological interventions to increase persulfidationassociate with increased longevity and improved capacity to cope with stress stimuli.

DOI: 10.1016/j.cmet.2019.10.007

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(19)30562-5

期刊信息

Cell Metabolism:《细胞—代谢》,创刊于2005年。隶属于细胞出版社,最新IF:22.415
官方网址:https://www.cell.com/cell-metabolism/home
投稿链接:https://www.editorialmanager.com/cell-metabolism/default.aspx