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科学家发现棕色脂肪与白色脂肪分泌组差异
作者:小柯机器人 发布时间:2019/10/25 15:37:34

德国马克斯·普朗克生物化学研究所Matthias Mann和丹麦哥本哈根大学Camilla Scheele小组,利用蛋白质组学的人类棕色和白色脂肪细胞分泌组比较图谱,揭示了EPDR1是一种新型的棕色脂肪因子(Batokine)。相关论文10月24日在线发表于国际学术期刊《细胞—代谢》。

研究人员对来自成人锁骨上棕色脂肪和皮下白色脂肪细胞的细胞培养基进行了高灵敏度质谱蛋白质组学研究。研究人员鉴定了471种潜在分泌蛋白质,这些蛋白涵盖了多种类别,例如激素、生长因子、细胞外基质蛋白质和补体系统的蛋白质,并且这些蛋白质在棕色和白色脂肪细胞之间存在差异调节。总共有101种蛋白质只在棕色脂肪细胞中表达,其中包含了室管膜蛋白相关蛋白1(EPDR1)。在人类血浆中检测到EPDR1的表达,并且功能研究表明,EPDR1在脂肪形成过程中发挥了决定性产热作用。

研究人员报道了人类棕色和白色脂肪细胞分泌组之间的实质性差异,并鉴定了在人类新陈代谢过程中发挥重要调节剂作用的新型候选棕色脂肪因子(Batokine)。

据介绍,白色脂肪组织分泌的脂肪因子在代谢和体内稳态中起作用,而针对棕色脂肪分泌组的探索较少。

附:英文原文

Title: Proteomics-Based Comparative Mapping of the Secretomes of Human Brown and White Adipocytes Reveals EPDR1 as a Novel Batokine

Author: Atul S. Deshmukh, Lone Peijs, Jacqueline L. Beaudry, Naja Z. Jespersen, Carsten H. Nielsen, Tao Ma, Andreas D. Brunner, Therese J. Larsen, Rafael Bayarri-Olmos, Bhargav S. Prabhakar, Charlotte Helgstrand, Mai C.K. Severinsen, Birgitte Holst, Andreas Kjaer, Mads Tang-Christensen, Annika Sanfridson, Peter Garred, Gilbert G. Privé, Bente K. Pedersen, Zachary Gerhart-Hines, Sren Nielsen, Daniel J. Drucker, Matthias Mann, Camilla Scheele

Issue&Volume: 2019/10/24

Abstract: Adipokines secreted from white adipose tissue play a role in metabolic crosstalk and homeostasis, whereas the brown adipose secretome is less explored. We performed high-sensitivity mass-spectrometry-based proteomics on the cell media of human adipocytes derived from the supraclavicular brown adipose and from the subcutaneous white adipose depots of adult humans. We identified 471 potentially secreted proteins covering interesting categories such as hormones, growth factors, extracellular matrix proteins, and proteins of the complement system, which were differentially regulated between brown and white adipocytes. A total of 101 proteins were exclusively quantified in brown adipocytes, and among these was ependymin-related protein 1 (EPDR1). EPDR1 was detected in human plasma, and functional studies suggested a role for EPDR1 in thermogenic determination during adipogenesis. In conclusion, we report substantial differences between the secretomes of brown and white human adipocytes and identify novel candidate batokines that can be important regulators of human metabolism.

DOI: 10.1016/j.cmet.2019.10.001

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(19)30556-X

期刊信息

Cell Metabolism:《细胞—代谢》,创刊于2005年。隶属于细胞出版社,最新IF:22.415
官方网址:https://www.cell.com/cell-metabolism/home
投稿链接:https://www.editorialmanager.com/cell-metabolism/default.aspx